Fenerbahce moved up into second place in their group following a 1-0 win over Celtic in the UEFA Europa League earlier this evening.
Van Persie had another frustrating night in-front of goal and was taken off midway through the second half.
Fernandao scored his sides winner after coming off the bench in place of Van Persie. Nani played the full 90 minutes for the Istanbul giants.
Former United striker Persie rose through the youth ranks at Feyenoord on his path towards becoming a professional.
Feyenoord are of course the rivals of Ajax and Van Persie would of wanted to score against his old foes. The experienced striker scored the winning goal in the 1-0 victory over Kayserispor on the weekend and already has five goals to his name this season.
Fenerbahce confirmed line-up: Fabiano, Caner Erkin, Ba, Simon Kjaer, Gokhan Gonul, Souza, Mehmet Topal, Diego, Luis Nani, Lazar Markovic, Robin van Persie
Ajax confirmed line-up: Cillessen, Van Rhijn, Veltman, Riedewald, Dijks, Bazoer, Gudelj, Klaassen, Schone, El Ghazi, Fischer