Bayer Leverkusen take on Roma at the Stadio Olimpico in the Champions League hoping close the gap with Group E leaders Barcelona.
Roma have a number of missing players including Totti and Keita while Florenzi is out.
Turkish international Salih Uçan is match fit but is unlikely to start for the Italian giants.
Bayer will be without Jedvaj (thigh), Hilbert (knee), Seung-Woo (ankle) due to injury while Bender (ankle) is a doubt.
Manchester United target Hakan Çalhanoğlu is almost certain to start.
The German tabloid Bild confirmed that the Red Devils sent scout to monitor the Turkish international during Bayer Leverkusen’s 4-4 draw with Roma in the UEFA Champions League a fortnight ago.
Roma v Leverkusen
Kick-Off: 19:45 GMT
Stadium: Stadio Olimpico
Roma: Szczęsny; Florenzi, Manolas, Rüdiger, Digne; Nainggolan, De Rossi, Pjanić; Gervinho, Džeko, Salah.
Out: Totti (thigh), Keita (thigh)
Doubtful: Florenzi (fatigue)
Leverkusen: Leno; Donati, Tah, Papadopoulos, Wendell; Kampl, Kramer; Mehmedi, Bellarabi; Hernández, Kiessling.
Out: Jedvaj (thigh), Hilbert (knee), Seung-Woo (ankle)
Doubtful: Bender (ankle)