AS Roma need to pull off a miracle tonight if they are to advance to the next stage of the UEFA Champions League.
The Serie A giants suffered a 4-1 defeat in the first league at the Camp Nou which mean they need to defeat Barca by three goals.
Tonight’s game takes place at the Olimpico stadium while kick-off is scheduled for 19:45 (GMT).
Turkey international Cengiz Under has been included in Roma’s squad after recovering from an injury which kept him out for the past month.
Meanwhile Barcelona boss Ernesto Valverde has opted to field Leo Messi, Suarez and Iniesta tonight. Summer signing Dembele starts the encounter on the bench as does Paulinho.
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AS Roma v Barcelona – Confirmed lineups
AS Roma Confirmed lineups: Alisson, Manolas Fazio, Jesus, Kolarov, Nainggolan,De Rossi, Strootman, Florenzi, Dzeko, Schick
Barcelona Confirmed lineups: Ter Stegen, Semedo, Pique, Umtiti, Jordi Alba, Sergio Roberto, Rakitic, Sergio, Iniesta, Suarez, Messi