Besiktas take on Bayern Munich in the Champions League Last 16 first-leg in Germany on 20 February.
The Black Eagles have made three changes to their squad heading into the tie.
Cenk Tosun has been removed after joining Everton on a £27 million move last month.
Orkan Cinar and Matek Mitrovic have also been taken off the squad list.
January arrivals Domagoj Vida, Vagner Love and Cyle Larin have all been added to the list submitted to UEFA.
Denys Boyke and Aras Ozbiliz who were not named in the group stage list have also been left out again.
Besiktas booked a place in the Last 16 after cruising through Group G as undefeated leaders.
The Istanbul giants beat Red Bull Leipzig, Porto and Monaco away from home scoring 11 goals in total while conceding just five times.
Tosun was involved in six of the Black Eagles 11 goals but they do have Anderson Talisca available who netted four times and Ryan Babel as well as Ricardo Quaresma who played an important contribution.
Besiktas are currently six-points behind league leaders Basaksehir in the Super Lig and recently booked a place in the Champions League Last 16.