Galatasaray take on Besiktas at the Vodafone Park stadium on Sunday in the Super Lig.
The Lions have not been in the best of form after failing to win their last two games.
Fans of the Yellow-Reds turned up in force to back their team in their final training session ahead of the derby clash.
41,504 paying supporters turned up in a show of support which set a new world record.
Imagine turning up to training and seeing this!
Scenes as 40,514 Galatasaray fans turned up to back their team ahead of the derby clash against Beşiktaş
Incredible ???#50BinAslanTekYürek pic.twitter.com/VHB4FZEhiB— TFTV Turkish-Football (@TurkFootballTV) December 1, 2018
The reigning Super Lig champions feel they were let down by the referee in the 1-1 draw against Konyaspor in the league last week.
The replays showed that the referee made a mistake in awarding Konyaspor a penalty which resulted in Serdar Aziz being sent off. The match official refused to look at VAR which would most likely have overruled the call.
Yellow-Reds fans have developed a siege mentality as a result with club officials and fan groups complaining that match officials and the authorities are singling them out.